Instrument centre for indirecte calorimetri and stable isotopes – University of Copenhagen

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08 March 2013

Instrument centre for indirecte calorimetri and stable isotopes

Instrument Centre for Indirecte Calorimetri and Stable Isotopes at the Department of Veterinary Clinical and Animal Sciences. 

Technical specification
The main facilities of the centre are located at the research station Rørrendergård, Tåstrup and consist of equipment for measuring of nutrient and energy metabolism at the whole body level:

Indirect calorimetry system - Respiration unit (open air-circuit princi-ple) with double chambers for animals from 1 to 5 kg body weight and double chambers for animals from 5 to 250 kg, for long-term measurements of gas exchange, substrate oxidation and energy expenditure.

Micro-calorimeter “Micro-Oxymax” (open and close air-circuit princi-ple) with 4 chambers for measurements of gas exchange and energy expenditure in embryos and small size animals from 1 to 500 g.

IRIS® Infra-Red Isotope Analyser System for measuring 13C in ex-pired air.

Equipment for digestibility and quantitative protein and energy balance studies with mice, rats, mink, foxes, dogs, poultry, pigs, goat, sheep, liamas, horses and cattle.


Use of equipment
The data, which can be achieved by indirect calorimetry and stable isotope measurements provides basic information about nutrient and energy metabolism at the whole body level. The following can be measured:

From indirect calorimetry and nutrient/energy balances:

  • Digestibility
  • Oxidation of protein, carbohydrate and fat
  • Energy expenditure
  • Retention of protein, fat and energy
  • Utilization of nutrients for different life processes under variety of fac-tors; genetic, health status, physical training, dietary composition feed/food additives, environmental conditions, etc.

 

From stable isotope technique:

  • Gastric Emptying
  • Liver- and Pancreas-Function
  • Lactase Deficiency
  • Helicobacter pylori,
  • Total heat production

From combined indirect calorimetry and stable isotope tech-niques:

  • Protein and energy metabolism
  • Amino acid decarboxylation and oxidation rates
  • Fatty acids oxidation, lipogenesis and lipolysis
  • Gluconeogenesis and glycogenesis
  • Glucose oxidation and glycogenolysis

Target Group

  • internal researcher
  • external researchers
  • companies
  • research partners