National High-Throughput DNA Sequencing Centre – University of Copenhagen

16 April 2013

National High-Throughput DNA Sequencing Centre

Technical specification
The danish National High-Throughput DNA Sequencing Centre offers a broad selection of next-generation sequencing data (Illumina and Roche/454 technologies), and provides whole genome optical mapping (OpGen) to improve sequence assembly and accelerate comparative genomics projects. It hosts a HiSeq2000, a HiSeq2500 and a MiSeq (Illumina), a GS FLX and a GS FLX+ (Roche/454) as well as an Argus Optical Mapping System (OpGen). We also have several dedicated powerful servers for data storage and analysis.

Use of equipment
Our staff can build a wide range of sequencing libraries (Illumina or Roche com-patible) from DNA (genomic, cDNA, PCR products), or can process prebuilt libraries. We provide quality control of the libraries (qubit, bioanalyzer, qPCR) and sequencing on our platforms. Raw data are provided as fastq files through a ftp.
We also deliver whole genome optical maps and temporary access to Genome Builder software (OpGen), based on DNA we extract from cell cultures or blood samples.

Target Group
• Researchers at the University of Copenhagen
• Permanent collaborators
• External users form other universities in Denmark and internationally
• External users from the industry

Additional information
please read more at our website.